LOCUS       40924_23769       12843 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB724 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12843)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 12843)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12843)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12843)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1178..1831
                     /codon_start=1
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             1838..1855
                     /codon_start=1
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
                     /translation="LVPRGS"
     protein_bind    1865..1989
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2026..2056
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2110..2766
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             3111..3413
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3457..3581)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3607..3859
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3893..3909)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3917..3933
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3941..3971)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3986..4007)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(4043..4295)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4333..5628)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61229.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(4333..5628)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(5680..5859)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(6365..6389)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6910..7698
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7947..8535
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8721..8861)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9205..9399)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9468..10538)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10970..11596)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcatg tcccctatac taggttattg
     1201 gaaaattaag ggccttgtgc aacccactcg acttcttttg gaatatcttg aagaaaaata
     1261 tgaagagcat ttgtatgagc gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt
     1321 gggtttggag tttcccaatc ttccttatta tattgatggt gatgttaaat taacacagtc
     1381 tatggccatc atacgttata tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga
     1441 gcgtgcagag atttcaatgc ttgaaggagc ggttttggat attagatacg gtgtttcgag
     1501 aattgcatat agtaaagact ttgaaactct caaagttgat tttcttagca agctacctga
     1561 aatgctgaaa atgttcgaag atcgtttatg tcataaaaca tatttaaatg gtgatcatgt
     1621 aacccatcct gacttcatgt tgtatgacgc tcttgatgtt gttttataca tggacccaat
     1681 gtgcctggat gcgttcccaa aattagtttg ttttaaaaaa cgtattgaag ctatcccaca
     1741 aattgataag tacttgaaat ccagcaagta tatagcatgg cctttgcagg gctggcaagc
     1801 cacgtttggt ggtggcgacc atcctccaaa atcggatctg gttccgcgtg gatccgctgt
     1861 tatcacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat
     1921 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
     1981 gtcactatgg cggcccctcg aggggccgca ttaggcaccc caggctttac actttatgct
     2041 tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag
     2101 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
     2161 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
     2221 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
     2281 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
     2341 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
     2401 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
     2461 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
     2521 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
     2581 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
     2641 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat
     2701 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
     2761 ggggcgtaaa cgcgtggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc
     2821 gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga
     2881 ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca
     2941 aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg
     3001 tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg
     3061 gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta
     3121 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata
     3181 ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag
     3241 ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga
     3301 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca
     3361 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt
     3421 caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt
     3481 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
     3541 tcattttacg tttctcgttc agctttcttg tacaaagtgg tgataaccta gagctcgaat
     3601 ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     3661 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     3721 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     3781 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     3841 tcatctatgt tactagatcg ggaattggtt ccggaaccaa ttcgtaatca tggtcatagc
     3901 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     3961 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaggc tgaattaggc
     4021 gcgcctattt ctattaattc ccgatctagt aacatagatg acaccgcgcg cgataattta
     4081 tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact
     4141 ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc
     4201 ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat
     4261 cttaagaaac tttattgcca aatgtttgaa cgatcgggga aattcgcctc gagtgacttc
     4321 tctgattgca gactatttat accaaccagc aagaaagtgt cgaagtatga agcagaaggc
     4381 gcaggctaga gcctggaaaa cgagaagatg gatgcaaaga gacttttctg atttaggacc
     4441 aaacaacctc aagtaaacat tgacgagtgt cccatctttt gtgccggtca gtaatccggt
     4501 agcaggatca taccttggga gacgatggcg cggacatttc acaatcccag caagctgagg
     4561 aagcgataat agaaggttca gaacttgagg aagaaagaag atgaggaggg tttcactgaa
     4621 gtgacccaga ataccaacaa ctgccatagt cattccggcg aagactgtgt aagtgtctcc
     4681 aacaaaaact gaagaaggat accaattgta agcaagcatt gccaaggatg tggccatcag
     4741 cggttgagta agaaatatcg agaaggcatg agcttggtga tactcgggat caacagatgc
     4801 tccgatttgc ataacgttgt gtatcaggat agcagcggca ataacaactg tttgaccgat
     4861 ttcaaggcca ttcagaccag cgtggatgtt aatagagttt gtacaaaaga cagcaagtag
     4921 ccccatatat aacttatata tccttcctag attaagcact tccaagccaa tataagcaac
     4981 aagaggtttt ggtataacaa tagttgtatg accagcataa gccatcaaaa gtggaagggt
     5041 agcaaaagat ggcaggacaa gttttactct ccaaggcaca tcaaggacat catctacaaa
     5101 tccaagcaaa atcatgaagc aaatagatgc tagtgctgca ttatactcca caagccaatt
     5161 agaatcttca gtgaagttaa aatactgaaa tattatcgcc acgatcaaaa agacaatacc
     5221 aacaacaata cccaatgact ctggcacttt aatatctcct tgaggcgtgc ctcttttgtt
     5281 gatatcaaaa ccaaacatgt tacgtctcag aacgtatctg gcagtaacag gaatcatttt
     5341 gagagtgacg aaaaacccaa ccaagctaag ccccgcgtta attagaatcg atcgcttgag
     5401 ttcattatca accttgtagt gaaaacagag aaggtataga tagagcgaac agagaagagt
     5461 agagatcacg aagatcactc ctaatttcgg cggcgcgaga cgaaattcct caccggaagc
     5521 agaaaccgtc tttcgggtca tcttctgctc cgatggcgct gagttgggtt ccgacgggtc
     5581 cggtttgttg ggaattgaga tcgatgaagc tctcttacga gctgccatat cttattgccc
     5641 cccgggatca gatctggatt gagagtgaat atgagactct aattggatac cgaggggaat
     5701 ttatggaacg tcagtggagc atttttgaca agaaatattt gctagctgat agtgacctta
     5761 ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt gcttagctca ttaaactcca
     5821 gaaacccgcg gctgagtggc tccttcaacg ttgcggttct gtcagttcca aacgtaaaac
     5881 ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc ccttaattct ccgctcatga
     5941 tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca
     6001 gggcttccca accttaccag agggcgcccc agctggcaat tccggttcgc ttgctgtcca
     6061 taaaaccgcc cagtctagct atcgccatgt aagcccactg caagctacct gctttctctt
     6121 tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca
     6181 ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt tagcagccct tgcgccctga
     6241 gtgcttgcgg cagcgtgaag ctgatccgtc gagattaata gaaataaatt cagcctaatt
     6301 cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca
     6361 atttgtttac accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc
     6421 tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga
     6481 gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa
     6541 ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg
     6601 atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac ataagcctgt
     6661 tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
     6721 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     6781 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     6841 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     6901 ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     6961 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     7021 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
     7081 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     7141 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     7201 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     7261 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     7321 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     7381 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     7441 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     7501 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     7561 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     7621 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
     7681 accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac gccgcttcgc
     7741 ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat
     7801 caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga
     7861 gatatatcat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7921 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7981 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8041 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     8101 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8161 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8221 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8281 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8341 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     8401 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     8461 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     8521 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     8581 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     8641 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     8701 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     8761 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     8821 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     8881 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     8941 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     9001 agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct
     9061 ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga
     9121 cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct
     9181 tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag agttttaata
     9241 agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac
     9301 atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca
     9361 atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct
     9421 ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat
     9481 gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc
     9541 tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa
     9601 cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat
     9661 ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg
     9721 ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg
     9781 atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg
     9841 ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg
     9901 ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag
     9961 gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg
    10021 tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg
    10081 ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg
    10141 gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc
    10201 acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg
    10261 cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg
    10321 cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg
    10381 cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt
    10441 tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg
    10501 ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg
    10561 ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg
    10621 ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc
    10681 cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt
    10741 agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac
    10801 atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc
    10861 gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca
    10921 cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg
    10981 atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt
    11041 caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg
    11101 actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg
    11161 tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa
    11221 tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct
    11281 gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct
    11341 ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa
    11401 tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat
    11461 cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca
    11521 gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta
    11581 cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata
    11641 cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg
    11701 gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa
    11761 accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta
    11821 cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc
    11881 gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg
    11941 tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc
    12001 acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca
    12061 gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt
    12121 gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc
    12181 ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg
    12241 gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc
    12301 aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg
    12361 gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc
    12421 ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg
    12481 cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg
    12541 cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc
    12601 tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag
    12661 ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac
    12721 ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta
    12781 ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa
    12841 cct
//
